DNA damage-related RNA expression to assess individual sensitivity to ionizing radiation

DNA damage-related RNA expression to assess individual sensitivity to ionizing radiation.

Predictive markers of intrinsic radiosensitivity in healthy individuals are needed in monitoring their occupational or environmental radiation exposure and may predict a patient's response to radiotherapy. Ionizing radiation can induce a large spectrum of DNA lesions, but under optimal DNA repair conditions, the principal residual lesions of importance are misrepaired double-strand breaks. The ...

Reduced DNA damage in tumor spheroids compared to monolayer cultures exposed to ionizing radiation

Background: Several cell lines when cultured under proper condition can form three dimensional structures called multicellular tumor spheroids. Tumor spheroids are valuable in vitro models for studying physical and biological behavior of real tumors. A number of previous studies using a variety of techniques have shown no relationship between radiosensitivity and DNA strand breaks in monolayer ...

Effect of Exposure to Ionizing Radiation on Biomarker of Oxidative Damage of DNA

Introduction: one of the most important complications of exposure to ionizing radiation is emergence of cancer tumors, which happens as a result of oxidative DNA.  Therefore, the present study was conducted, aimed to measuring 8-DIHYDROxy- 2’- DEOXYGUANOSINE (8-OHdG) level in radiographers’ urine as oxidative damage biomarker, as well as comparing this biomarker with cumulative effective doses....

Chromatin modifications and the DNA damage response to ionizing radiation

In order to survive, cells have evolved highly effective repair mechanisms to deal with the potentially lethal DNA damage produced by exposure to endogenous as well as exogenous agents. Ionizing radiation exposure induces highly lethal DNA damage, especially DNA double-strand breaks (DSBs), that is sensed by the cellular machinery and then subsequently repaired by either of two different DSB re...

The DNA-Damage Response to Ionizing Radiation in Human Lymphocytes